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The effect of pyrazine derivatives on secondary metabolites content in plant cultures in vitro - III.
Blahnová, Kristýna ; Tůmová, Lenka (advisor) ; Karlíčková, Jana (referee)
One of the possibilities how to increase the production of secondary metabolites (SM) in plant in vitro cultures is the method of elicitation. The aim of this work was to determine the effect of an abiotic elicitor from the class of pyrazine derivatives 1-benzyl-3-(pyrazin-2-yl)urea on the production of flavonolignans of silymarin complex and flavonoid taxifolin in the plant culture Silybum marianum (L.) Gaertn. Pyrazine derivatives are investigated for their herbicidal properties, so elicitation proceeds by the mechanism of stress inducing effect. Tissue cultures were grown on Murashige and Skoog growth medium with the addition of the growth regulator α-naphthylacetic acid. Elicitation was performed on both callus and suspension cultures. The elicitor was used in three different concentrations: c1 = 100.0 mg/100 ml; c2 = 10.0 mg/100 ml; c3 = 1.00 mg/100 ml. Particular samples were taken after 6, 24, 48, 72 and 168 hours of elicitor effect, control samples after 6, 48 and 168 hours. After drying, the callus and suspension tissues were extracted with methanol and the content of the monitored secondary metabolites was determined by HPLC. It was also tested if SM are released into the growth medium. Flavonolignans silybinin A and silybinin B were not detected in any of the analyzed samples. The...
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Analysis of milk thistle (\kur{Silybum marianum}) crop management in agricultural company
ŠINDELKOVÁ, Tereza
This diploma thesis deals with monitoring of milk thistle cultivation in selected farm in South Bohemia. The growth of the milk thistle was followed by fees for assessing growth and its developmental phase. Pre-harvest plant selections to estimate the number of flowers on the leaves and the number on the flowers. The feed yield, the tissue content for feeding, the water and dry matter content, the fat and N content, the total antioxidant activity were determined.
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The effect of pyrazine derivatives on secondary metabolites content in plant cultures in vitro - III.
Blahnová, Kristýna ; Tůmová, Lenka (advisor) ; Karlíčková, Jana (referee)
One of the possibilities how to increase the production of secondary metabolites (SM) in plant in vitro cultures is the method of elicitation. The aim of this work was to determine the effect of an abiotic elicitor from the class of pyrazine derivatives 1-benzyl-3-(pyrazin-2-yl)urea on the production of flavonolignans of silymarin complex and flavonoid taxifolin in the plant culture Silybum marianum (L.) Gaertn. Pyrazine derivatives are investigated for their herbicidal properties, so elicitation proceeds by the mechanism of stress inducing effect. Tissue cultures were grown on Murashige and Skoog growth medium with the addition of the growth regulator α-naphthylacetic acid. Elicitation was performed on both callus and suspension cultures. The elicitor was used in three different concentrations: c1 = 100.0 mg/100 ml; c2 = 10.0 mg/100 ml; c3 = 1.00 mg/100 ml. Particular samples were taken after 6, 24, 48, 72 and 168 hours of elicitor effect, control samples after 6, 48 and 168 hours. After drying, the callus and suspension tissues were extracted with methanol and the content of the monitored secondary metabolites was determined by HPLC. It was also tested if SM are released into the growth medium. Flavonolignans silybinin A and silybinin B were not detected in any of the analyzed samples. The...
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The effect of abiotic elicitors on secondary metabolites content in plant cultures in vitro - II.
Tomaidesová, Barbora ; Tůmová, Lenka (advisor) ; Karlíčková, Jana (referee)
The Effect of Abiotic Elicitors on the Content of Secondary Metabolites in In vitro Plant Cultures - II. The method of elicitation is used for the increased production of secondary metabolites in in vitro plant cultures. In this study, there the effect of pyridine derivate N-(5-chlorpyridin-2-yl)-4-ethylbenzamide in three concentration - 3.845.10-3 mol/l; 3.845.10-4 mol/l and 3.845.10-5 mol/l for the production of flavonolignans in callus and suspension cultures of Silybum marianum was tested. The evaluation of elicitation effect proceeded after 6, 24, 48, 72 a 168 hours against control samples without the elicitor treatment after 24 and 168 hours. In the same intervals the samples of culture medium were taken and assessed. The cells were cultivated on Murashige and Skoog medium with 10 mg/ml of α-naphtylacetic acid as growth regulator. The obtained and adjusted samples were analysed by the HPLC method. From the parts of silymarin complex were detected silychristin, silybin A, isosylibin A, isosilybin B. Flavonoid taxifolin wasn̕ t detected in any case. In callus culture, there was achieved a maximal production of all detected components of silymarin complex (30.508 μg/g DW) after 168 hours of elicitor treatment in concentration of 3.845.10-4 mol/l, it was about only substance - silychristin. The...
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Zkrmování výlisků ostropestřce mariánského u nosnic na začátku snášky
Čaganová, Soňa
This thesis is aimed at studying the effect of the addition of milk thistle (Silybum marianum) seed cakes to the diet on metabolism, liver function and performance of laying hens at the beginning of laying. In the experiment, the results were compared between a group of laying hens, which were fed with the basic feed mixture without the addition of milk thistle seed cakes with the experimental group with the addition of milk thistle seed cakes in the feed mixture. It was situated 30 hens breed ISA Brown into the experiment, divided into two groups. The first group was experimental with the addition of milk thistle seed cakes (7 % in the mixture), and the second group was a control. The experiment evaluated egg laying, egg weight, food consumption and the animal weight. During the experiment, blood was twice collected from animals for biochemical examination focusing on the parameters of liver profile. The results of the experiments didn´t show any effects of feeding milk thistle seed cakes on the body weight of laying hens. By evaluating the performance parameters of the laying hens it showed a significantly lower egg laying, but also showed a lower consumption of feed (P < 0.05). At the same time, the data found higher weigh of eggs (P < 0.01) in this group, but the egg production was significantly lower than in the control group. During the examination of biochemical parameters of blood there was a significantly higher concentration of bilirubin, uric acid and cholesterol (P < 0.05) in the blood plasma of laying hens fed by the addition of milk thistle seed cakes. At the second sampling (approximately 2 months) the laying hens that were fed with milk thistle seed cakes have consistently higher concentration of cholesterol (P < 0.05) and lower activity of dehydrogenase, creatine kinase (P < 0.05) and uric acid (P < 0.01) in the blood. In the experiment there was no clear positive or negative effects of the milk thistle seed cakes on the performance parameters and biochemical profile of the blood plasma.
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Vliv elicitorů, hnojení a technologie pěstování Ostropestřce mariánského (Silybum marianum L) na produkt a jeho využití
GUBIŠOVÁ, Jana
Milk Thistle (Silybum marianum) has been one of the best known medici herb for a very long time. Seeds contain biologicky aktive substances taxifoiln, silychristin, silydianin, silybin A, silybin B, isosilybin A and isosilybin B, commonly known as silymarin complex. The aim of this thesis was the influence of elicitors, fertilization and growing technology on this product and its utilizaion. In the first part I focused on history, botanic attributes, agrotechnology, chemical composition and substance efficiency method of determinativ and also on pharmacological onpacts of effective substances. In the practical part I conducted twho small parcel experiments to reveal the impal of elicitors on effective substances. I used two elicitors NanoFYTSi? in 1ml/l concentrantion and N-FENOLMIX? in 0.5ml/l concentration. In the second experiment from my supervisor elicitor NanoFYT Si? in 1ml/l concentraiton and N-FENOL MIX? in 0.5ml/l and elicitor ASA were used in three different concentration: low [10-5 mol/l], medium [ 10-4 mol/l] and high [10-3 mol/l]. In the conclusion I did statistical analysis of the above montioned experiments and compared them with other Publisher experiments. Then I propřed the growing technology and utilization of Milk Thistle.
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Vliv sylimarinu na jatečnou výtěžnost králíků
KREJČÍKOVÁ, Klára
The work deals with the influence of the Milk thistle (used substance Silymarin) on the carcass yield of hybrid combinations rabbits Hyla. Hybrid combination Hyla was purposefully bred for meat performance, showing excellent results in breeding. Rabbits behave primarily on meat, because nutritional properties correspond to the current requirements of rational nutrition. Therefore, it is appropriate to increase the carcass yield. The average slaughter yield is indicated from 40 to 53% according to the breed. It was found that the addition of milk thistle in the form of a powder in the complete feedingstuff at a concentration of 0.2% increased the carcass yield, relative to the control group (which was fed only KKS), on average by 0.4%. The group with the addition of 1% showed an average value of 1.2% higher than the group control. The average slaughter yield of all the groups ranged from 54,68 into 63,02%. In both farms also decreased the incidence of diarrheal disease. Silymarin has a favorable effect on the operation and growth of the liver. In the group with the addition of 0.2% increased the average liver weight of 8.1 g, in the group with a concentration of 1% weight on average picked up about 10g. The average weight of the liver varied from 111,19 to 121,20 g. The control group in the case of carcass yield and liver weight showed better results than the authors of their articles.
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Growing technology of milk thistle (Silybum marianum L. Gaertn.) and fertilization with the aim of maximizing the quality of the product and its use
GUBIŠOVÁ, Jana
Milk thistle (Silybum marianum L. Gaertn.) a medicinal plant known for thousands years. In the Czech Republic is purposely planted since the 70s of last century. Last few years increased worldwide interest about this plant mainly due to the pharmaceutical industry, which uses isolated of the active ingredients. The advantage of this plant is low demands on environment and high yields if suitable agrotechnics aspects and soil quality are provided. The main objective of this thesis was to gather available information about milk thistle, growing technology, and familiarity with its botanical characteristics, chemical composition and active ingredients, farming techniques, use, fertilization and past management. Furthermore the study was focused on summarizing the methods for the determination of certain active substances clarification the pharmacological effects and the influence of elicitation and cultivation techniques for the active substances. In the conclusion was summarized the problem of the cultivation of milk thistle and technology designed for growing in practice and of its use. Especially as a technology of soil preparation, choosing suitable proceeding crop, seeding, fertilizing and the most demanding plant harvest. Furthermore there was given a proposal for the use of seeds of milk thistle in the food industry, where is considered as insufficient. The potential of this medicinal plant would be for example for the production of functional foods.
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Meat yield of rabbits
KREJČÍKOVÁ, Klára
This bachelor thesis deals with a topic of meat yield of crossbred rabbits. The basis assumption of quality of meat production, fur and rabbit skin is a sufficient nutrition as well as genetic disposal. The nutrition and feed depend on breeding and housing technology. Nowadays many types of feed exist. The most important fact in feeding is the time. It is necessary to gain the slaughter weight as soon as possible. In the first experimental group the slaughter weight (2600 g) was gained in 70th day of rabbit's life no matter if it was female or male animal. But in the second one the slaughter weight was gained after 77 days. The increases in weeks of feeding were equal, differences in the first groups were inconclusive, and in the second one there was a difference of 15, 5 g/week (2, 2 g/day) between female and male animals. The consumption of feed was different in those two experimental groups. In the first group there was no evidence of different volume of feed between female and male animals whereas in the second one the female animals consumed more feed than male animals. The difference was 4, 3 g/day. Silybum marianum was added to the feeding mixture in concentration 1, 0 % in the first group and in 0, 2 % in the second one.
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